Technologies for large-scale physical mapping of human chromosomes
نویسندگان
چکیده
منابع مشابه
Physical mapping of human chromosomes by repetitive sequence fingerprinting.
We have developed an approach for identifying overlapping cosmid clones by exploiting the high density of repetitive sequences in complex genomes. Individual clones are fingerprinted, using a combination of restriction enzyme digestions followed by hybridization with selected classes of repetitive sequences. This "repeat fingerprinting" technique allows small regions of clone overlap (10-20%) t...
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Successful isolation, derivation and culturing of human pluripotent stem cells, including human embryonic stem cells (hESCs) and human induced pluripotent stem (hiPSCs) cells in laboratory scale has opened new horizones for cell therapy applications such as tissue engineering and regenerative medicine. However, most of the cell therapy protocols using these unique cells require large number of ...
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Physical map reconstruction in the presence of er rors is a central problem in genetics of high compu tational complexity A parallel genetic algorithm for a maximum likelihood estimation based approach to physical map reconstruction is presented The estima tion procedure entails gradient descent search for de termining the optimal spacings between probes for a given probe ordering The optimal p...
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Reconstructing a physical map of a chromosome from a genomic library presents a central com putational problem in genetics Physical map reconstruction in the presence of errors is a prob lem of high computational complexity A parallel evolutionary method for a maximum likelihood estimation based approach to physical map reconstruction is presented The estimation procedure entails gradient desce...
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A new study led by the International School for Advanced Studies (SISSA) of Trieste and published in PLOS Computational Biology adds detail to the theoretical models used in chromatin simulations and demonstrates that even when made up of a mixture of fibres with different properties chromatin does not alter its three-dimensional structure above a certain spatial resolution. This finding points...
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ژورنال
عنوان ژورنال: Laboratory Automation & Information Management
سال: 1996
ISSN: 1381-141X
DOI: 10.1016/s1381-141x(96)80005-0